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Pathogen

Epicoccum nigrum

Overview

Scientific name

Epicoccum nigrum
Genus

Epicoccum
EPPO code

PHOMEC
Common name

Leaf spot of cereals
Synonyms

Epicoccum purpurascens

Description

From ISTA's ' Common Laboratory Seed Health Testing Methods for Detecting Fungi':

The fungus produces brown to black sporodochia, usually in groups. Sporodochia appear rough and granular, containing numerous small, brownish black, more of less spherical conidia on short and stout conidiophores, 15-25 μm. The conidial wall is verrucose to tubercular or areolate. 

Images of Epicoccum nigrum

Epicoccum nigrum on Triticum spp.
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Epicoccum nigrum on Triticum spp.

Image credits: Nicole Calliou, CA

Colonies are usually white to red to green on top, and always red on rear. Yellowish stain produced in PDA agar around colony.

sterilisation: 1% NaOCl 4 min | growth time: 5 days | temperature: 25C -/+2C | lighting: 3:1 (fluorescent:nUV), within 60cm of light. Alternating light/dark in 12 hr intervals | plate size: 100mm | agar: PDA ( Neogen) |
DNA testing: None

Epicoccum nigrum on Triticum spp.
click for more information

Epicoccum nigrum on Triticum spp.

Image credits: Nicole Calliou, CA

Colonies are usually white to red to green on top, and always red on rear. Yellowish stain produced in PDA agar around colony.

sterilisation: 1% NaOCl 4 min | growth time: 5 days | temperature: 25C -/+2C | lighting: 3:1 (fluorescent:nUV), within 60cm of light. Alternating light/dark in 12 hr intervals | plate size: 100mm | agar: PDA ( Neogen) |
DNA testing: None

Epicoccum nigrum on Triticum spp.
click for more information

Epicoccum nigrum on Triticum spp.

Image credits: Nicole Calliou, CA

Colonies are usually white to red to green on top, and always red on rear. Yellowish stain produced in PDA agar around colony.

sterilisation: 1% NaOCl 4 min | growth time: 5 days | temperature: 25C -/+2C | lighting: 3:1 (fluorescent:nUV), within 60cm of light. Alternating light/dark in 12 hr intervals | plate size: 100mm | agar: PDA ( Neogen) |
DNA testing: None

Epicoccum nigrum on Lens culinaris
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Epicoccum nigrum on Lens culinaris

Image credits: Nicole Calliou, CA

Colony is typically white to red to green on top, and always has a red reverse, with yellow staining surrounding colony on PDA. Spores are rounded and bumpy.

sterilisation: 1% NaOCl 4 min | growth time: 7 days | temperature: 25C -/+2C | lighting: 3:1 (fluorescent:nUV), within 60cm of light. Alternating light/dark in 12 hr intervals | plate size: 100mm | agar: PDA ( Neogen) |
DNA testing: None

Epicoccum nigrum on Lens culinaris
click for more information

Epicoccum nigrum on Lens culinaris

Image credits: Nicole Calliou, CA

Colony is typically white to red to green on top, and always has a red reverse, with yellow staining surrounding colony on PDA. Spores are rounded and bumpy.

sterilisation: 1% NaOCl 4 min | growth time: 7 days | temperature: 25C -/+2C | lighting: 3:1 (fluorescent:nUV), within 60cm of light. Alternating light/dark in 12 hr intervals | plate size: 100mm | agar: PDA ( Neogen) |
DNA testing: None

Epicoccum nigrum on Lens culinaris
click for more information

Epicoccum nigrum on Lens culinaris

Image credits: Nicole Calliou, CA

Colony is typically white to red to green on top, and always has a red reverse, with yellow staining surrounding colony on PDA. Spores are rounded and bumpy.

sterilisation: 1% NaOCl 4 min | growth time: 7 days | temperature: 25C -/+2C | lighting: 3:1 (fluorescent:nUV), within 60cm of light. Alternating light/dark in 12 hr intervals | plate size: 100mm | agar: PDA ( Neogen) |
DNA testing: None