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Pathogen

Didymella pinodes

Overview

Scientific name

Didymella pinodes

Genus

Didymella

EPPO code

MYCOPI

Common name

Blight

Synonyms

Ascochyta pinodes

Description

From APS Press and CMI description #340: 

Pycnidia are freely formed on many media, and may be embedded in the medium, or distributed on the surface. They are globose and brown or black, and open by papillate ostioles. The conidiophore, conidia, or pycnidiospores are (3-6.5 x 8-16 μm, average 4.5 x 12.3 um) hyaline, typically with one septum (some 2 or 3) that has a slight to prominent constriction, and ellipsoid. Profuse dark brown or olive-coloured chlamydospores are mainly intercalary and in chains.  Colonies are light to dark grey, often growing with concentric rings. The principle characteristic differentiating the three Asochyta spp. is the presence of perithecia in D. pinodes. The conidia of D. pinodella are distinctly smaller and more spherical than those of A. pisi. The conidial dimensions of D. pinodes and D. pinodella overlap; however the absence of septa in a large portion of D. pinodella is also a distinguishing feature. 

Images of Didymella pinodes

Didymella pinodes on Pisum sativum
click for more information

Didymella pinodes on Pisum sativum

Image credits: Ruud Barnhoorn, 0

What you see are pea seed under the binocular with lighting below 2.5X zoomed. A clear brown colony with an umbrella of brown spore structures can be observed.


sterilisation: 1% NaOCL-oplossing 10 minutes | growth time: 9 days | temperature: 20 +/- 2C | lighting: 12 hrs of NUV and 12hrs in darkness | plate size: 90mm | agar: PDA + streptomycin ( Millipore (Merck)) |
DNA testing: -

Didymella pinodes on Pisum sativum
click for more information

Didymella pinodes on Pisum sativum

Image credits: Ruud Barnhoorn, 0

What you see are pea seed under the binocular with lighting below 2.5X zoomed. A clear brown colony with an umbrella of brown spore structures can be observed.


sterilisation: 1% NaOCL-oplossing 10 minutes | growth time: 9 days | temperature: 20 +/- 2C | lighting: 12 hrs of NUV and 12hrs in darkness | plate size: 90mm | agar: PDA + streptomycin ( Millipore (Merck)) |
DNA testing: -