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Pathogen

Didymella pinodella

Overview

Scientific name

Didymella pinodella

Genus

Didymella

EPPO code

PHOMMP

Common name

Foot rot

Synonyms

Ascochyta pinodella

Description

Didymella pinodella, previously Ascochyta pinodella, and then called Phoma medicaginis var. pinodella, causes foot rot of peas. Often found co-infecting pea seeds with Ascochyta pinodes and Ascochyta pisi. 

From APS Press and CMI description # 518: 

Pycnidia are globose and brown or black. Conidia (2-3 x 4.5-8 μm) are smaller than those of D. pinodes, hyaline, 1- or 2-septate and oval-cylindric with rounded edges. Profuse dark chlamydospores are both intercalary and terminal and are borne singly and in chains. Optimal temperatures for pycnidium and spore production on PDA are 18-24C. Light is required for conidial germination. Colonies are dark grey, and many become black at maturity. Some isolates appear slick because of the predominance or oozing pycnidia and chlamydospores over the mycelium. The principle characteristic differentiating the three Asochyta spp. is the presence of perithecia in D. pinodes. The conidia of D. pinodella are distinctly smaller and more spherical than those of A. pisi. The conidial dimensions of D. pinodes and D. pinodella overlap; however the absence of septa in a large portion of D. pinodella is also a distinguishing feature. 

Images of Didymella pinodella

Didymella pinodella on Pisum sativum
click for more information

Didymella pinodella on Pisum sativum

Image credits: Ruud Barnhoorn, 0

What you see are on the plate are pea seed with different fungi growing out of them, the dark brown fungi (circled in blue) is to be considered as A. pinodella.


sterilisation: 1% NaOCL-oplossing 10 minutes | growth time: 9 days | temperature: 20 +/- 2C | lighting: 12 hrs of NUV and 12hrs in darkness | plate size: 90mm | agar: PDA + streptomycin ( Millipore (Merck)) |
DNA testing: -

Didymella pinodella on Pisum sativum
click for more information

Didymella pinodella on Pisum sativum

Image credits: Ruud Barnhoorn, 0

What you see are on the plate are pea seed with different fungi growing out of them, the dark brown fungi (circled in blue) is to be considered as A. pinodella.


sterilisation: 1% NaOCL-oplossing 10 minutes | growth time: 9 days | temperature: 20 +/- 2C | lighting: 12 hrs of NUV and 12hrs in darkness | plate size: 90mm | agar: PDA + streptomycin ( Millipore (Merck)) |
DNA testing: -

Didymella pinodella on Pisum sativum
click for more information

Didymella pinodella on Pisum sativum

Image credits: Ruud Barnhoorn, 0

What you see are on the plate are pea seed with different fungi growing out of them, the dark brown fungi (circled in blue) is to be considered as A. pinodella.


sterilisation: 1% NaOCL-oplossing 10 minutes | growth time: 9 days | temperature: 20 +/- 2C | lighting: 12 hrs of NUV and 12hrs in darkness | plate size: 90mm | agar: PDA + streptomycin ( Millipore (Merck)) |
DNA testing: -