Clavibacter michiganensis
Overview
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Scientific name
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Clavibacter michiganensis |
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Genus
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Clavibacter |
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EPPO code
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CORBMI |
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Common name
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Bacterial Canker |
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Synonyms
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Clavibacter michiganensis subsp. michiganensis |
Description
From ISTA Working Sheet #67:
On SCM agar, after 10 days growth, colonies are convex, irregular, mucoid with internal black flecks.
On mSCM agar, after 7 days growth, colonies are light gray, 2-3 mm in diameter, translucent, and easily distinguishable from other mucoid colonies by the presence of internal flecks (specks). As incubation time increases, the colonies become larger and the internal flecks become yellow.
Subculture suspected colonies onto YDC agar, looking for yellow mucoid colonies. Confirm identity with IF staining, ELISA or pathogenicity test.
Images of Clavibacter michiganensis
Clavibacter michiganensis on Solanum lycopersicum
Image credits: Ruud Barnhoorn, 0
Dilution plating of seed extract of 10.000 tomato seed on Cmm1 medium. Seed extract created by soaking seed in phosphate buffer overnight, stationary @4C. Extracted after incubation by 4min of stomaching.
sterilisation: - - | growth time: 10 days | temperature: 28+/-2C | lighting: darkness | plate size: 90mm | agar: Cmm1 ( Duchefa) |
DNA testing: -
Clavibacter michiganensis on Solanum lycopersicum
Image credits: Ruud Barnhoorn, 0
Dilution plating of seed extract of 10.000 tomato seed on Cmm1 medium. Seed extract created by soaking seed in phosphate buffer overnight, stationary @4C. Extracted after incubation by 4min of stomaching.
sterilisation: - - | growth time: 10 days | temperature: 28+/-2C | lighting: darkness | plate size: 90mm | agar: Cmm1 ( Duchefa) |
DNA testing: -
Clavibacter michiganensis on Solanum lycopersicum
Image credits: Ruud Barnhoorn, 0
Dilution plating of seed extract of 10.000 tomato seed on SCMF medium. Seed extract created by soaking seed in phosphate buffer overnight, stationary @4C. Extracted after incubation by 4min of stomaching.
sterilisation: - - | growth time: 10 days | temperature: 28+/-2C | lighting: darkness | plate size: 90mm | agar: SCMF ( Duchefa) |
DNA testing: -
Clavibacter michiganensis on Solanum lycopersicum
Image credits: Ruud Barnhoorn, 0
Dilution plating of seed extract of 10.000 tomato seed on SCMF medium. Seed extract created by soaking seed in phosphate buffer overnight, stationary @4C. Extracted after incubation by 4min of stomaching.
sterilisation: - - | growth time: 10 days | temperature: 28+/-2C | lighting: darkness | plate size: 90mm | agar: SCMF ( Duchefa) |
DNA testing: -
Clavibacter michiganensis on Solanum lycopersicum
Image credits: Marion Jousselin, FR
Colonies of Clavibacter michiganensis (Cmmt plates)
growth time: 10 days | temperature: 28°C | lighting: dark | agar: Cmmt ( ) |
DNA testing: Reference isolate
Clavibacter michiganensis on Solanum lycopersicum
Image credits: Marion Jousselin, FR
Colonies of Clavibacter michiganensis (Cmmt plates)
growth time: 10 days | temperature: 28°C | lighting: dark | agar: Cmmt ( ) |
DNA testing: Reference isolate
Clavibacter michiganensis on Solanum lycopersicum
Image credits: Marion Jousselin, FR
Colonies of Clavibacter michiganensis (Cmmt plates)
growth time: 10 days | temperature: 28°C | lighting: dark | agar: Cmmt ( ) |
DNA testing: Reference isolate
Clavibacter michiganensis on Solanum lycopersicum
Image credits: Marion Jousselin, FR
Colonies of Clavibacter michiganensis (Scmf plates)
growth time: 10 days | temperature: 28°C | lighting: dark | agar: Scmf ( ) |
DNA testing: Reference isolate
Clavibacter michiganensis on Solanum lycopersicum
Image credits: Marion Jousselin, FR
Colonies of Clavibacter michiganensis (Scmf plates)
growth time: 10 days | temperature: 28°C | lighting: dark | agar: Scmf ( ) |
DNA testing: Reference isolate
Clavibacter michiganensis on Solanum lycopersicum
Image credits: Marion Jousselin, FR
Colonies of Clavibacter michiganensis (Scmf plates)
growth time: 10 days | temperature: 28°C | lighting: dark | agar: Scmf ( ) |
DNA testing: Reference isolate