Stemphylium spp.
Overview
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Scientific name
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Stemphylium spp. |
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Genus
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Stemphylium |
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EPPO code
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STEMSP |
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Common name
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Stemphylium leaf spot |
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Synonyms
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Description
Commonly identified by the distinctive spores. Described in the ISTA 'Common Laboratory Seed Health Testing Methods for Detecting Fungi' as conidia being smooth walled, a few minutely echunulate, and/or verrucose, and appearing as rpundish square to rectangular packets of different sizes, with both transverse and longitudinal septa, pale to dark brown.
Images of Stemphylium spp.
Stemphylium spp. on Lens culinaris
Image credits: Nicole Calliou, CA
Lobate colony, typically with a green-ish top and brown reverse with darker spots where perithecia are forming. Conidia are square-ish, brown, and have both transverse and longitudinal septae.
sterilisation: 1% NaOCl 4 min | growth time: 7 days | temperature: 25C -/+2C | lighting: 3:1 (fluorescent:nUV), within 60cm of light. Alternating light/dark in 12 hr intervals | plate size: 100mm | agar: PDA ( Neogen) |
DNA testing: None
Stemphylium spp. on Lens culinaris
Image credits: Nicole Calliou, CA
Lobate colony, typically with a green-ish top and brown reverse with darker spots where perithecia are forming. Conidia are square-ish, brown, and have both transverse and longitudinal septae.
sterilisation: 1% NaOCl 4 min | growth time: 7 days | temperature: 25C -/+2C | lighting: 3:1 (fluorescent:nUV), within 60cm of light. Alternating light/dark in 12 hr intervals | plate size: 100mm | agar: PDA ( Neogen) |
DNA testing: None
Stemphylium spp. on Lens culinaris
Image credits: Nicole Calliou, CA
Lobate colony, typically with a green-ish top and brown reverse with darker spots where perithecia are forming. Conidia are square-ish, brown, and have both transverse and longitudinal septae.
sterilisation: 1% NaOCl 4 min | growth time: 7 days | temperature: 25C -/+2C | lighting: 3:1 (fluorescent:nUV), within 60cm of light. Alternating light/dark in 12 hr intervals | plate size: 100mm | agar: PDA ( Neogen) |
DNA testing: None
Stemphylium spp. on Brassica spp.
Image credits: Nicole Calliou, CA
The perithecia of Stemphylium on Canola are profuse on this sample (on blotter).
sterilisation: None N/A | growth time: 7 days | temperature: 25C -/+2C | lighting: 3:1 (fluorescent:nUV), within 60cm of light. Alternating light/dark in 12 hr intervals | agar: Blotter paper moistened with water ( N/A) |
DNA testing: None