Fusarium culmorum
Overview
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Scientific name
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Fusarium culmorum |
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Genus
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Fusarium |
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EPPO code
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FUSACU |
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Common name
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Cereal foot rot and head blight |
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Synonyms
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Fusisporium culmorum |
Description
On PDA, growth is rapid, with dense aerial mycelium, generally white but often yellow to tan on the base. The undersurface is orange to carmine red. Orange to red brown sporodochia appear readily.
On malt agar, the mycelium is short, pink to carmine red. Sporodochia form on older cultures and are orange to red brown in colour.
Conidiophores: Unbranched and branched monophialides.
Macroconidia are stout, distinctly septate (typically 3-4 septate), thick walled, and have straight to curved ventral and dorsal surfaces. The basal cells is usually distinctly round and can occasionally be foot-shaped (rare), to slightly notched. Macroconidia are usually uniform in shape and size. Microconidia are absent.
Chlamydospores generally form abundantly and quickly, they may occur singly, in chains, or in clumps. Typical growth time is 3-5 weeks on CLA for formation.
From ISTA's Common Laboratory Seed Health Testing Methods for Detecting Fungi:
The fungus produces abundant dull orange to light brown, slimy pionnotes, variable in shape and size, often covering the whole seed.
Images of Fusarium culmorum
Fusarium culmorum on Triticum spp.
Image credits: Nicole Calliou, CA
Typical fast growing Fusarium culmorum colony showing the orange coloration common on certain brands of PDA. Different brands can influence the coloration to be darker, almost red. Image 3 shows a close up of sporodochia which are also orange in color. Spores are commonly mistaken for F. crookwellense or F. sambucinum, but F. culmorum are usually 3-4 septate with a notched basal cell (no 'foot' shape), and the spores are very wide (often compared to maggots in our lab).
sterilisation: 1% NaOCl 4 min | growth time: 5 days | temperature: 25+/-2C | lighting: 3:1 (fluorescent:nUV), within 60cm of light. Alternating light/dark in 12 hr intervals | plate size: 100mm | agar: PDA ( Neogen) |
DNA testing: None
Fusarium culmorum on Triticum spp.
Image credits: Nicole Calliou, CA
Typical fast growing Fusarium culmorum colony showing the orange coloration common on certain brands of PDA. Different brands can influence the coloration to be darker, almost red. Image 3 shows a close up of sporodochia which are also orange in color. Spores are commonly mistaken for F. crookwellense or F. sambucinum, but F. culmorum are usually 3-4 septate with a notched basal cell (no 'foot' shape), and the spores are very wide (often compared to maggots in our lab).
sterilisation: 1% NaOCl 4 min | growth time: 5 days | temperature: 25+/-2C | lighting: 3:1 (fluorescent:nUV), within 60cm of light. Alternating light/dark in 12 hr intervals | plate size: 100mm | agar: PDA ( Neogen) |
DNA testing: None
Fusarium culmorum on Triticum spp.
Image credits: Nicole Calliou, CA
Typical fast growing Fusarium culmorum colony showing the orange coloration common on certain brands of PDA. Different brands can influence the coloration to be darker, almost red. Image 3 shows a close up of sporodochia which are also orange in color. Spores are commonly mistaken for F. crookwellense or F. sambucinum, but F. culmorum are usually 3-4 septate with a notched basal cell (no 'foot' shape), and the spores are very wide (often compared to maggots in our lab).
sterilisation: 1% NaOCl 4 min | growth time: 5 days | temperature: 25+/-2C | lighting: 3:1 (fluorescent:nUV), within 60cm of light. Alternating light/dark in 12 hr intervals | plate size: 100mm | agar: PDA ( Neogen) |
DNA testing: None
Fusarium culmorum on Triticum spp.
Image credits: Nicole Calliou, CA
Typical fast growing Fusarium culmorum colony showing the orange coloration common on certain brands of PDA. Different brands can influence the coloration to be darker, almost red. Image 3 shows a close up of sporodochia which are also orange in color. Spores are commonly mistaken for F. crookwellense or F. sambucinum, but F. culmorum are usually 3-4 septate with a notched basal cell (no 'foot' shape), and the spores are very wide (often compared to maggots in our lab).
sterilisation: 1% NaOCl 4 min | growth time: 5 days | temperature: 25+/-2C | lighting: 3:1 (fluorescent:nUV), within 60cm of light. Alternating light/dark in 12 hr intervals | plate size: 100mm | agar: PDA ( Neogen) |
DNA testing: None
Fusarium culmorum on Hordeum vulgare subsp. vulgare
Image credits: SASA, GB
Fusarium culmorum from Hordeum vulgare seeds on Oxoid PDA, front and back images
sterilisation: NaOCl (1% available chlorine) 10 minutes | growth time: 7 days | temperature: 20C +/- 2C | lighting: Darkness | plate size: 90mm | agar: PDA ( Oxoid )
Fusarium culmorum on Hordeum vulgare subsp. vulgare
Image credits: SASA, GB
Fusarium culmorum conidia
Fusarium culmorum on Hordeum vulgare subsp. vulgare
Image credits: SASA, GB
Fuarium culmorum sporodochia on carnation leaf on CLA
growth time: 3 | temperature: 20C +/- 2C | lighting: Darkness and supplementary NUV at 360nm to encourage sporulation | plate size: 90mm | agar: CLA (Oxoid Technical agar no3 with carnation leaves) ( Oxoid )