Xanthomonas euvesicatoria pv. perforans on Solanum lycopersicum
Image credits: Ruud Barnhoorn, 0
Dilution plating of seed extract of 10.000 tomato seed on mMXV medium. Seed extract created by soaking seed in phosphate buffer overnight, stationary @4C. Extracted after incubation by 4min of stomaching. Different/inconsistent sizes of the target pathogen are clearly visible in the pictures. These inconsistencies in size are a result of them coming from seed extract and different growth stages they are in. Please be aware of this verification of suspect colonies by molecular techniques is highly recommended.
sterilisation: - - | growth time: 7 days | temperature: 28+/- 2C | lighting: darkness | plate size: 90mm | agar: mMXV ( duchefa) |
DNA testing: -
Xanthomonas euvesicatoria pv. perforans on Solanum lycopersicum
Image credits: Ruud Barnhoorn, 0
Dilution plating of seed extract of 10.000 tomato seed on mMXV medium. Seed extract created by soaking seed in phosphate buffer overnight, stationary @4C. Extracted after incubation by 4min of stomaching. Different/inconsistent sizes of the target pathogen are clearly visible in the pictures. These inconsistencies in size are a result of them coming from seed extract and different growth stages they are in. Please be aware of this verification of suspect colonies by molecular techniques is highly recommended.
sterilisation: - - | growth time: 7 days | temperature: 28+/- 2C | lighting: darkness | plate size: 90mm | agar: mMXV ( duchefa) |
DNA testing: -
Xanthomonas euvesicatoria pv. perforans on Solanum lycopersicum
Image credits: Ruud Barnhoorn, 0
Dilution plating of seed extract of 10.000 tomato seed on mMXV medium. Seed extract created by soaking seed in phosphate buffer overnight, stationary @4C. Extracted after incubation by 4min of stomaching. Different/inconsistent sizes of the target pathogen are clearly visible in the pictures. These inconsistencies in size are a result of them coming from seed extract and different growth stages they are in. Please be aware of this verification of suspect colonies by molecular techniques is highly recommended.
sterilisation: - - | growth time: 7 days | temperature: 28+/- 2C | lighting: darkness | plate size: 90mm | agar: mMXV ( duchefa) |
DNA testing: -
Xanthomonas euvesicatoria pv. perforans on Solanum lycopersicum
Image credits: Ruud Barnhoorn, NL
Dilution plating of seed extract of 10.000 tomato seed on mTMB medium. Seed extract created by soaking seed in phosphate buffer overnight, stationary @4C. Extracted after incubation by 4min of stomaching. A white cloudy halo (as clearly visible at the bottom picture) is expected surrounding the yellow XantPF colony. in the top picture this is less clearly visible.
sterilisation: - - | growth time: 7 days | temperature: 28+/- 2C | lighting: darkness | plate size: 90mm | agar: mTMB ( duchefa) |
DNA testing: -
Xanthomonas euvesicatoria pv. perforans on Solanum lycopersicum
Image credits: Ruud Barnhoorn, NL
Dilution plating of seed extract of 10.000 tomato seed on mTMB medium. Seed extract created by soaking seed in phosphate buffer overnight, stationary @4C. Extracted after incubation by 4min of stomaching. A white cloudy halo (as clearly visible at the bottom picture) is expected surrounding the yellow XantPF colony. in the top picture this is less clearly visible.
sterilisation: - - | growth time: 7 days | temperature: 28+/- 2C | lighting: darkness | plate size: 90mm | agar: mTMB ( duchefa) |
DNA testing: -
